Abstract:A new enzyme sensor for determination of dioleglyceride was constructed by nano-nickle oxide modified through electro- chemical oxidation sedimentary reaction on the screen-printied electrode (SPE) and lipase immobilized by chitosan. Using ferricyanide as the electron mediator, , the CV behavior of dioleglyceride on lipase-NiO-SPE were evalutaed in the solution of 1% Triton-X100 and PBS buffer (0.1 mol/L, pH 7.0). A good linear relationship was obtained between dioleglyceride ranging in 0.04~180 μmol/L with cyclic voltammetry (R =0.993) and detection limit of 0.01 μmol/L. This method was simple, rapid, accurate, and steady for the determination of dioleglyceride.
