Clone and Analysis ζ- Carotene Desaturase of Dunaliella bardawil
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Abstract:
ζ-Carotene desaturase (ZDS) is one of the key enzymes in the carotenoid biosynthetic pathway. According to the full-length sequence of the ZDS cDNA in D. bardawil that our laboratory has obtained before, the coding region sequence of ZDS were isolated by the approach of PCR and then flanking sequence of the coding region were amplified by the method of genome walking, including promoter and terminator of ZDS, followed by analysis of these sequences by bioinformatics tools. The whole sequence of ZDS was11896 bp. It contained 6435 bp coding region (from ATG to TAA), 4091 bp upstream of the coding region, and 1370 downstream of the coding region. Blastn the coding region and ORF of ZDS showed that there were 12 exons and 11 introns in coding region. The total length of the introns was 4086 bp, about 2.68 fold of the total length of exons (1749bp). Each intron started with GT and end with AG, belonging to the most common style of exon. By analyzing the promoter of ZDS gene, we found that there are many transcription factor binding sites, including “GAGA box”, ASF1, related with plant light regulation; “ACGT box”, required for etiolation-induced expression of early responsive to dehydration.
