Isolation and Identification of the Lactic Acid Bacteria from Infant Faeces and their Therapeutical Effect on Diarrhea of Mice
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Abstract:
Six Gram-positive bacteria with strong acidogenicity were separated and purified from fresh feces of healthy babies, and their bacteriostasis in vitro were tested. Three strains with high inhibition effect on diarrhoeic Escherichia coli (O78, O88 and O149) were screened. The results of biochemical identification and 16S rRNA comparison analysis showed that all the three screened strains were Lactobacillus casei. The challenged test in mice and chickens by one strain with strongest inhibition on pathogenic E. coli showed that the strain was safe, nontoxic and appropriately improved the animal feed conversion rate, which can used for prevention and treatment of diarrhea effect.
