Abstract:A Blumlein type pulse forming network system was developed for treating Vibrio parahaemolyticus suspension solution. The results showed a high disinfection ability of this high voltage pulse electric fields on Vibrio parahaemolyticus. Meanwhile, the cytotoxicity of the bacteria was reduced after the bacteria were treated by this system. The expression level of three type secretion system -related genes increased with the increase of voltage, indicating that high-voltage pulse electric fields affected the pathogenic of bacteria. This research could be a theoretical base for liquid food disinfection, bioproducts and vaccine inactivation.