Abstract:Advantage spoilage bacteria were identified in chilled duck by traditional bacteria isolation method and 16S rDNA flora analysis. 17 advantage spoilage bacteria had been detected in chilled duck by traditional bacteria solation method. 16S rDNA flora analysis showed that dominant spoilage bacteria in chilled ducks was of Pseudomonas, being of 54%. Strains of Aeromonas and Enterobacter were the second groups accounted for 15%. The content of a strans of Lactobacillus, Arthrobacter or Bacillus, as well as an unidentified strains,was accounted for 4%.