Rapid Detection of Listeria monocytogenes in Milk Powder by Loop-mediated Isothermal Amplification
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Abstract:
Loop-mediated isothermal amplification (LAMP) is a novel DNA amplification technology with high specificity, efficiency and rapidity under isothermal conditions. In this research, a set of specific LAMP primers were designed to amplify the specific hlyA gene of Listeria monocytogenes by LAMP technology. The results showed that the detection sensitivity and detection limit of the LAMP method for purely cultured Listeria monocytogenes were 2.45×101 cfu/mL and 7.32×101cfu/mL, respectively. For other food pathogens, the target bands were not found . This method was suitable for rapid detection of Listeria monocytogenes in contaminated foods.
