Abstract:KOH catalyzed transesterification of inferior cooking oil was investigated for biodiesel production. The results showed that biodiesel production yield was increased at first and then decreased with increasing the reaction temperature, which reached the maximum value 92.6% at 65℃. The biodiesel yield can reached 89.2% when reaction time was of 1.5h. Further increasing the reaction time above 1.5 h led to only slightly increase in the biodiesel yield. Ratio of methanol to oil has a slight effect on biodiesel production and the optimal KOH dosage was 0.8%, with which 92.9% of biodiesel yield can be obtained.