Optimization of Ultrasonic-assisted Enzymatic Extraction of Polysaccharide from Phellinus igniarius
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Abstract:
Ultrasonic-assisted extraction was applied to improve the cellulase-catalyzed extraction yield of polysaccharide from Phellinus igniarius.Based on the single factor experiments, four factors and three levels of response surface analysis method was adopted to optimize the extraction process based on central composite design principle. Infrared spectrometry was used to analyze the structural characteristics of the extracted polysaccharide from Phellinus igniarius. Results showed that the suitable extraction processing conditions were ultrasonic treatment temperature 54℃, pH 5.1, ultrasonic treatment time 39 min and ultrasonic treatment power 240w. Under the optimum conditions, the yield of Phellinus igniarius polysaccharide was up to 5.30%. Infrared spectroscopy indicated that the observed major functional groups in Phellinus igniarius polysaccharide were consistent with those extracted by hot water.
