Optimization of Extraction Conditions for Polysaccharides from Collybia albuminosa via Response Surface Methodology
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Abstract:
The extraction technology of polysaccharides from Collybia albuminosa was optimized via response surface methodology (RSM). Using extraction rate of polysaccharide from Collybia albuminosa as the indicator, effects of extraction temperature, time, water-material ratio and extraction times on the extraction rate of polysaccharide were investigated. Based on single factor studies, a three-variable, three-level Box-Benhnken experimental design and response surface methodology were used to obtain the optimized extraction conditions of polysaccharides. The optimum conditions of extraction technology of polysaccharide from Collybia albuminosa were extraction temperature of 87 ℃, extraction time of 2.9 h and water-material ratio of 31:1 (mL/g), under which the extraction rate of polysaccharide was up to 16.88%.
