Abstract:An analytical method for the determination of isocarbophos residues in cowpea with HPLC/MS/MS was established. Samples were extracted by acetonitrile with sonic extracting and detected LC/MS/MS. The LODs (S/N=3) were 0.1 μg/kg. For spiking level at 0.1, 1.0, and 5.0 μg/kg, the average recoveries were 80.1%~87.9% and RSD (n=6) values ranged from 6.7% to 8.1%. Using the MRM-IDA-EPI model, MRM chromatograms and the MS2 mass spectrum of the samples can be obtained by only one single injection. The method was simple, precise and had high recoveries, which can meet the requirement for residues analysis.
