A Specific Loop-mediated Isothermal Amplification Method for Detection of Transgenic Maize MON89034
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Abstract:
According to MON89034 exogenous insert sequences connection with the plant genome sequences, the best specific primers were designed, the reaction system and reaction conditions were optimized, and finally a loop-mediated isothermal amplification method for detecting transgenic maize MON89034 was established. Specificity, sensitivity, stability and repeatability of this method were tested. The results showed that the method can specifically detect maize MON89034, and the detection sensitivity of the method was up to 1pg.With the maize MON89034 DNA samples of 1.00%, 0.10%, 0.05% concentration as templates, stability and repeatability testing was conducted, false negative rate was 0. The results showed that the LAMP method is suitable for specifically detecting transgenic maize MON89034.
