Abstract:A novel high performance liquid chromatography (HPLC) method was developed for the analysis of imidaeloprid pesticide residue in fresh vegetables. Imidaeloprid was extracted from the samples by acetonitrile, purified with LC-NH2 column and determined by high performance liquid chromatography with UV detection. Symmetry Shield? C18 column (4.6×250 mm, 5 μm) using the mixture of methanol, water and acetonitrile (55:25:20) as the mobile phase. The column temperature, flow rate，detection wavelength and sample volume were 30.0 ℃, 0.8 mL/min, 275 nm and 10.0 μL, respectively. Within the linear range of 0.1~1.0 mg/L, the correlation coefficient, the recoveries of imidaeloprid in tomato and the relative standard deviations (RSD) were 0.9925, 95.4%~106.8 % and 2.66%~7.99%, respectively. The method was simple, effective and accurate. It can be applied to determinate imidaeloprid pesticide residue in fresh vegetables.
