Capability and Application of PMA-qPCR Method for Monitoring the Ultrasonic Sterilization Rate
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Abstract:
To evaluate the capability of PMA-qPCR method to monitor the sterilization rate of ultrasound, the cultures were treated with ultrasound and then subjected to PMA-qPCR or plate counting. The dates showed that the PMA-qPCR method was quite effective and applicable. Evaluation of the influence of different factors on the sterilization rate showed that the treatment time and the power were the most important parameters at certain temperature and ultrasonic frequency, while the duty ratio showed little influence on the sterilization rate.
