Effects of Nitrogen Source on Photosynthesis and Pigment Accumulation of Chlorella sp. TCCC45058
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Abstract:
In the study, effects of four kinds of nitrogen source (carbamide, NH4NO3, (NH4)2SO4 and NaNO3) on biomass, photosynthesis and Chlorophyll A productivity of Chlorella sp. TCCC45058 under autotrophy condition was investigated. The highest cell density (4.1×107/mL) of Chlorella sp. TCCC45058 was acquired when NaNO3 was used as nitrogen source. The optimal nitrogen source for pigment accumulation of Chlorella sp. TCCC45058 was carbamide, with which the highest Chlorophyll A productivity of cell was found as 21 mg/g. Different nitrogen source showed varied influences on medium pH. When NaNO3 was used as nitrogen source, medium pH rose significantly with consuming of NO3-. Use of (NH4)2SO4 as nitrogen source resulted in reduced medium pH. Carbamide and NH4NO3 had little effect on it. When medium pH was controlled at neutral, the result of culture has not improved. Therefore, it was unnecessary to adjust medium pH throughout the cultivation.
