Rapid Detection of Atrazine Residue in Water and Corn by Colloidal Gold-based Immunochromatographic Strip
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Abstract:
An immunochromatographic strip assay using colloidal gold-labeled antibody was developed. The visual limit of detection (LOD) of the assay for atrazine in PBS was 2 ng/mL. And the results could be obtained within 10 min. Spiked tap water sample was analyzed directly by the test strip without any pretreatment and the LOD was 2 ng/mL. Meanwhile, spiked corm sample was analyzed after simple pretreatment and the LOD was 40 ng/g. The results obtained by the immunochromatographic strip assay correlated well with those determine by indirect competitive ELISA. Because of its low cost, simple operation, high specificity, fast detection and easy-to-judgment results, the strip could serve as an on-site screening of a large number of samples.
