Abstract:The optimization of the extraction process of protein from Chlorella was achieved by response surface methodology. The optimization strategy used was a series of one-factor-at-a-time investigations and subsequent response surface analysis based on a quadratic regression rotary combination design. Results showed that Liquid-to-solid ratio of 21:1, pressure of 170 MPa and cycling times of 4 were found optimum for protein extraction from Chlorella. Under these optimum conditions, the yield of protein was 45.78%.
