Separation and Purification of Total Flavones from Lycium barbarum Leaves by Macroreticular Resin
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Abstract:
Purification of total flavones of Lycium Chinese leaves using macroreticular resins was investigated. Dynamic elution ratio of AB-8, ADS-5, ADS-7, ADS-17, ADS-21, and HP-20 were studied respectively and compared in order to find the optimum macroreticular resin. Results indicated that the HP-20 resin was the best due to its highest adsorption and desorption properties. The optimum conditions were: ratio of flavonoid volume to the bed volume of 13 BV, the pH value of sample 6, concentration of sample 2.336 mg/mL, current velocity 2 BV/h, 70% alcoholas eluant for desorption, current velocity 2B V/h and eluant volume 3.5 BV. Under those conditions, and the content of Lycium Chinese leaves flavonoids increased from 29.02% to 83.75%. The technique condition is scientific and reasonable. It can be used to separate and purificate total flavonoids from Lycium Chinese leaves. Flavonoid compounds in leaves of Lycium bararum were analyzed by using HPLC. Lutein content greatly increased from 9.88%to 23.79% after the column purification.
