Abstract:The fermentation process parameters of CHU-R, such as medium, incubation temperature, inoculation, etc. were discussed in this paper. It was found that the best medium formulas was: 6% sucrose, 1% molasses, 0.5% ammonium sulfate, 0.3% Na2HPO4，0.05% KI，and 0.05% MgSO4?7H2O. The optimum fermentation conditions in 50 L fermenter were: temperature 28℃, venti1ation 0.1~0.15 (V/V/min), the initial speed of 120rpm, tank pressure 0.05~0.08 MPa, liquid volume 30 L, the initial pH 6.8~7.0 and inoculum size 6%, the fermentation process to maintain dissolved oxygen at 40-60%. The results showed that the CHU-R yield pigment nearly reached 23 mg/g of its dry weigh fermented in 50 L fermenter.
