Effect of High Pressure on Aggregation Behavior of Kidney Bean (Phaseolus Vulgaris L) Protein Isolate
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Abstract:
The influence of high pressure (HP) treatment (200-600 MPa) on molecular distribution of kidney bean (Phaseolus vulgaris L) protein isolate (KPI) was investigated by High-performance size-exclusion chromatography combined with multi-angle laser light scattering technique (HPSEC-MALLS), to explore the possible HP-induced protein association-dissociation behavior. The molecular weight (Mw) of phaseolin, the major component in KPI, calculated based on the Debye plot, was about 161 kDa. HP treatment at 200 MPa led to dissociation of soluble aggregates (at void volume), while HP treatment at 400 and 600 MPa led to dissociation of insoluble aggregates in KPI, thus improved PS.
