Abstract:Three detecting methods for starch in tobacco were compared by parallel test and standard recovery. Results showed relative standard deviation and the recoveries of starch of the acid hydrolyzation was 2.26% and 107.86%, respectively. Those of the iodine coloration methods were 1.0397% and 98.45%, respectively. For continuous flow methods, the relative standard deviation and recoveries of starch were 1.33% and 98.63%, respectively. The accuracy of acid hydrolyzation was worse than the other two methods. The accuracy of iodine coloration was close to the cont inuous,, thus both of the two methods can be adapt to detect starch in tobacco.