Abstract:A simple and rapid method was developed for determination of 30 specified glucocorticosteroids. Sample was extracted and purified by three phases solvent system comprised of saturated aqueous sodium chloride solution, acetonitrile and hexane. After emulsified matrix was precipitated by potassium ferrocyanide and zinc acetate solution, 30 specified glucocorticosteroids were determined by Ultra Performance Liquid Chromatography (UPLC). In addition, extraction conditions were further optimized by systematically reseaching on effects of different extracting solvents and methods. With this method, the average recoveries for glucocorticosteroids ranged from 89.8 % to 105.2 %; The relative standard deviations were between 5.0 % and 10.3 %; And the method quntification limit was 2.0 mg/kg.
