Abstract:Jinjiang oyster (Crassostrea Rivularis) were hydrolyzed by the endogenous proteases to produce amino acid nitrogen and the influencing factors were tested. The optimum conditions were ascertained as pH 4, temperature 40 or 50 ℃, material/total ratio 0.5 and autolysis time 12 h. Three crucial parameters of Jinjiang oyster protein autohydrolysis, including temperature, initial pH value and material/total ratio, were optimized by using the response surface methodology. Results showed that the optimum autohydrolysis conditions were temperature 56.24 ℃, initial pH 4.00, material/total ratio 0.50 for 12 h, under which the actual value of amino nitrogen content reached up to 5.73 mg/mL, which was in close agreement with the predicted value of 5.81 mg/mL and higher by 39.42% than that without optimization.
