Abstract:The objective of this study was to explore technique of enzymatic hydrolysis of corn stalk pulp (removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase (enzyme activity: 13.94 FPU/100ml). The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47% (m/V), pH value of 4.70， temperature of 50℃ and enzymatic reaction time of 72 h. The highest yield of sugar was 59.41% under the optimum process conditions. The paper also presented the different enzymolysis of corn stalk pretreated by different methods, and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base > corn stalk pulp from alkaline cooking >> corn stalk pretreated by mechanical crushing > raw corn stalk.
