Optimization of Enzymatic Hydrolysis of Poecilobdella manillensis Lesson for Preparing of Ancoagulant Peptide
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Abstract:
In order to obtain high activity ancoagulant peptide from Poecilobdella manillensis Lesson, an enzymolysis method was established through sequential steps of screening single-factor and designing orthogonal test. By selecting ancoagulant activity as index, the optimum enzymolysis reaction conditions were determined as enzymolysis temperature 55℃, enzymolysis temperature 2h, substrate concentration 12%, enzyme dosage 3% and pH8.5. The ancoagulant activity could reach 702AT-U/g under the optimized conditions.
