Identification of the Strain BS-3 and Preliminary Study on the Characterization of the Antifungal Substance
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Abstract:
A strain of inhibition to many kinds of plant pathogenic fungi, named BS-3, was isolated from soils. The strain had quite strong fungistatic activities on plant pathogenic fungi such as rice sheath blight, rice blast. It had 100% homologous to Bacillus licheniformis, Bacillus subtilis and Bacillus amyloliquefaciens by 16S rDNA sequences BLAST analysis plus the results of morphological and biochemical parameters. It was confirmed as a strain of Bacillus licheniformis. Using rice sheath blight fungus Thanatephorus cucumeris as the indicator bacteria, inhibition zone method was chosen to detect antifungal activity of fermentation product belong to the strain BS-3. The results showed that the fermentation liquor after the treatment of centrifuging and 50% saturated ammonium sulfate had quite strong fungistatic activities. In addition, the antifungal substances had strong thermostability.
