Application of Loop-mediated Isothermal Amplification Assay for Detection Peanut allergy
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Abstract:
More and more attention is paid to peanut allergies, so the detection of peanut allergens is becoming increasingly important. In most case, antigen-antibody method and PCR method are mostly used in the detection of peanut allergen. However, antigen-antibody method takes longer, and the PCR method requires expensive equipment. In this report, the loop-mediated isothermal amplification (LAMP) method was developed to detect the gene of peanuts which is related to allergens producing. This method is rapid, simple and sensitive, which can provide convenience for peanut allergens detection. The establishment of the LAMP method has great significance and it provide a great convenience for food safety testing.
