Abstract:In this paper, reverse phase HPLC-ELSD and normal phase HPLC-RI were used to determine the products from glycerolysis of soybean oil. The results indicated that the two methods were suitable for determination of the relative content of monoacylglycerols (MAG), diacylglycerols (DAG) and triacylglycerols (TAG). Oleic acid glycerides were determined by reverse phase HPLC-UV, the standard curves of 1-monoolein (1-O), 1,3-diolein (1,3-OO) and triolein (OOO) were established, and the correlation coefficients were higher than 0.99.
