Abstract:Lipase-catalyzed synthesis of monoacylglycerol production between Linseed oil and glycerol in a solvent system by Lipozyme 435 was studied by response surface methodology. The result showed the optimal parameters were as follows: substrate molar ratio of Linseed oil to glycerol 1:3, the t-butyl alcohol ratio 39.66% (oil to t-butyl alcohol, m/V), enzyme dosage 6.01wt%, reaction temperature 56.11℃ and reaction time 10.48h. Under the optimal conditions, monoacylglycerol content could reach 71.1% in the reaction product, and the content of α-linolenic acid reached 51.36% in the monoacylglycerol.
