Lipase Immobilization with a New Crosslinking Reagent Tris(hydroxymethyl)phosphine
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Abstract:
In the present work, tris(hydroxymethyl)phosphine (THP) was used as a new crosslinking reagent in lipase immobilization on cotton towel with adsorbent polyethylenimine (PEI). The optimal conditions of immobilization by single factors test were obtained as follows: 0.5 mL of PEI (3.37 mg/mL, pH 7.0) for each 0.2 g cotton towe, 2ml of THP [0.10% (V/V)], immobilization temperature 40 ℃ and crosslinking reaction time 20 min. THP-immobilized lipase can catalyze interesterification of vinyl butyric acid and pentanol. The conversion rate in 1h achieved 92.1%, and the activity of immobilized lipase was 15.80 U/g. Compared with the GA-immobilized lipase, THP-immobilized lipase had higher reusability, thermal stability and activity, indicating this is a low cost and practical newly method for lipase immobilization.
