Evaluation of Inactivation Efficiency of Shigella bacteriophage by Aerosol Spray
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Abstract:
The efficiency of shigella phages mixture to inhibit pathogenic Shigella spp. by aerosol spray was investigated. Concentration of phages after aerosol spray was determined. Then, the host of Shigella flexneri 2a, Shigella dysenteriae and Shigella sonnei were sprayed with a total concentration of 3×105 cfu/mL. Phage cocktail were added thereafter (3×108 pfu/mL) immediately, and the number of Shigella spp. were counted 1 h, 2 h and 3 h respectively after spraying. The results showed that Shigella phages SF-2A、SD-11 and SS-92 could effectively inactivate their host strain, the host strain couldn’t be detected after spraying phages 3 h later. Therefore, the results suggest that phage cocktail, such as the phage of S. flexneri, S. dysenteriae and S. sonnei, could effectively reduce potential contamination of Shigella spp. in food production and processing.
