Abstract:A new method for determination of rutin and quercetin contents in buckwheat was developed in this paper. The extracts from buckwheat by Ultrasonic extraction was analyzed on a Shimadzu ODS column（4.6 mm * 150 mm, 5 μm）with a mobile phase of acetonitrile - water (containing 2% glacial acetic acid) for gradient elution at 360nm. And the flow rate was 1.0 mL/min. The linear ranges of rutin and quercetin concentrations were found within 0.023~1.150 μg (r = 0.9996) and 0.02~0.60 μg (r = 0.9999), respectively. The average recoveryies of rutin and quercetin were 98.5% and 95.8%, respectively. The method was simple, accurate and showed good reproducibility, which was suitable for the determination of rutin and quercetin contents in buckwheat.
