Extraction of Glycosaminoglycan from the Whole Viscera of Sanguinolaria acut
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Abstract:
The extraction of glycosaminoglycan from the whole viscera of the Sanguinolaria acuta was studied by single factor experiment. And the extracts was preliminarily purified and identified by electrophoresis. The optimal conditions for the enzymatic extraction of glycosaminoglycan from Sanguinolaria acuta were determined as follows: papain dosage 0.6%, subtilisin dosage 0.79%, the ratio of purified water to material 1.0:6, enzymolysis temperature 58 ℃, and enzymolysis time 4 h. Crude glycosaminoghycan (SAG-0) was achieved with the yield of 2.58%. After enzymatic extraction, deproteinization by centrifugation, ethanol precipitation, washing and drying, SAG-0 was achieved with the glycosaminoglycan content of 26.7%. Then SAG-0 was purified by decoloration, isoelectric precipitation, hyperfiltration, ethylalcohol precipitation, washing and drying, giving glycosaminoglycan superfine products (SAG-1) with the yield rate and glycosaminoglycan content being of 1.48% and 40.1%, respectively.
