Fermentation of Glutathione by Saccharomyces cerevisiae
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Abstract:
Fermentation of glutathione was studied through the ways of batch fermentation and fed-batch fermentation by YF（ZnCl2r，Ethr）, a test strain, which is mutant of Saccharomyces cerevisiae. The results show that the optimal fermentation condition: culture temperature 30 ℃, pH 6.0, inoculation 20%，rotate speed 150 r/min，ventilation 250 L/h. The effects of shake-flask culture and fermentor culture on GSH synthesis were studied in fed-batch fermentation. Conditions for the shake-flask culture methods are: initial sugar 10 g/L, fermentation at 4 h, 10 h, 16 h, 22 h, 28 h, respectively, glucose 25 g/L, 15 g/L, 5 g/L, 5 gL, 5 g/L added in fermentation solution, the final total glucose reached 65 g/L, the maximum cell dry weight reached 12.75 g/L, and the maximum GSH concentration and intracellular GSH content reached 76.49 mg/L and 0.59% respectively. The conditions for fermentor culture methods are: initial sugar 20 g/L, 12 h fermentation of sugar begans to fill up to 24 h, the final total glucose reached 160 g/L, the maximum cell dry weight reached 30.95 g/L, the maximum GSH concentration and intracellular GSH content were 68.49 mg/L and 0.3% respectively.
