Abstract:The antimicrobial effect of pure polysaccharide from walnut kernel pellicle (WKPP-70-1-1) with different concentration on E.coli was studied and its minimum inhibitory concentration (MIC) was determined as 400 μg/mL. Analysis of growth curve of E.coli effected by MIC of WKPP-70-1-1 showed that WKPP-70-1-1can inhibit the growth of E.coli, especially the logarithm phase of E.coli. After a certain time, the inhibition effect of WKPP-70-1-1 might disappear. The morphology of E.coli treated by WKPP-70-1-1 was observed by Atomic force microscopy and the results showed that smooth and no-wrinkle E.coli became wrinkles, rough, loss of the rules of morphology.
