Abstract:A quantitive assay for the determination of Ionosine-5'-monophosphate (5’-IMP) and Guanosine-5-monophosphate (5’-GMP) in pork was established by high performance liquid chromatography (HPLC). The separation was performed on a ZOBAX–NH2 column using methanol-NaH2PO4 (0.05 mol/L) as mobile phase and using a photo-diode arraydetector as detector at 260 nm. The linear range of the method was 1.00~100 μg/mL with the RSD and average recovery of 0.5%~3% and 85%, respectively. The method was accuracy, sensitivity and feasibility to analyze the nucleotides in pork.
