HPLC Fingerprint of Extracts of Taraxacum Bacteriostatic by Chloroform
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Abstract:
Caffeic acid was used as internal standard for the HPLC fingerprint establishment of chloroform extract from Taraxacum bacteriostatic. HPLC analysis was performed on a Diamonsil C18 column (5 μm, 4.6 mm×250 mm) with the three-phase system of acetonitrile-water-10% acetic acid solution as the mobile phase for gradient elution. The flow rate, detection wavelength and column temperature were 0.7 mL/min, 323 nm and 37 ℃, respectively. 12 Common peaks were showed in the fingerprint, which can be used to control the quantity of the Taraxacum bacteriostatic extraction.
