Inhibitory Effect of SEA on S180 Growing in C57BL/6 Mouse and Analysis of the Tumor Necrosis Factor
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Abstract:
This experiment studies the anti-tumor effect of the staphylococcal enterotoxin A on S180 solid tumor in C57BL/6 mouse modle and detect the anti-tumor rate and the spleen index. And the TNF-α content in the spleen and blood serum was also studied through ELISA and immunohistochemistry. S180 cells was injected to the oxter of the C57BL/6 to build up the solid tumor mouse model, then physiologic saline (control) and SEA (test) were intraperitoneally injected to these mouse every two days. Nine days later, the mousse were dissected to detect anti-tumor rate, spleen index and TNF-α .It was found that the weights of solid tumor in the test group (0.52±0.10 g) were lighter than the control group (1.02±0.11 g) (p<0.05). The spleen indexes of the two groups were15.9 and 4.8, respectively. Results also showed that TNF-α content in the test group was more than that in the control group. It was concluded that SEA can effectively improve the immunity of the mouses, thus killing the tumor cells in mouse model group.
