Application of Metabolic Flux Analysis for Screening of L-Histidine-Producing Strains
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Abstract:
According to the theory of metabolic flux analysis, TL1106(5-MTr,SGr,5-FTr,8-AGr,6-MPr,2-TAr, Hisase-) was obtained by diethyl sulfate (DES) mutagenesis derived from the original strain Corynebacterium glutamicum TQ2223 (Phe-,Tyr-,8-AGr,SGr,CINr). TL1106 can produce 6.0 g/L of L-Histidine in 10% glucose medium which was 2.5 times higher than that byTQ2223. Accordingly, the metabolic flux of C.glutamicum TQ2223 and its mutants TL1106 was determined, compared and analyzed in L-Histidine synthesis. Therefore, the great change of metabolic flux distribution might be caused by the introduction of genetic markers, which was useful in L-Histidine synthesis.
