Abstract:In this paper, the preservation of the mycelium of Aspergillus niger by freeze drying was introduced ,and the effects of the cultural medium and freeze-drying protectant were investigated. The enrichment culture medium for Aspergillus niger with high viable cells number and the best freeze-drying protectant were determined, with which the viable cells number of Aspergillus niger treated by freeze drying were 109cfu/g.