Optimization of the Extraction Technology of Polysaccharides from Pueraria by Response Surface Analysis
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Abstract:
The extraction of polysaccharides from the residue of pueraria after extraction of Flavonoids was studied in this paper. According to the single-factor experiments, three levels of the factors (extraction temperature, time and the ratio of water to pueraria residue) were selected for the central composite design to establish a quadric regression equation for describing the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction temperature of 98℃, time of 2.4 h and water-pueraria residue ratio of 33:1, under which the highest yield of polysaccharides reached with estimated value and verified value being of 12.59% and 12.63%, respectively.
