Studies on Isolation and Characteristics of Acidic Mucopolysaccharide from Holothuria arenicola Sempe
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Abstract:
Crud acidic mucopolysaccharide was extracted from enzymatic hydrolyzate of Holothuria arenicola Sempe by papain and then discolored with H2O2. After removing the protein with KAc, the mucopolysaccharidefurther was further purified by column chromatography, and analyzed by agarose electrophoresis. The Mw of this mucopolysaccharide was 76,008, and the content of sulfate, glucuronic acid, galgactosamine and fucose in the mucopolysaccharide were 28.08%, 20.71%, 15.38%, and 12.48% respectively.