Scavenging Activity of Enzymatic Hydrolysate of Yak Casein on DPPH Radical
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Abstract:
The DPPH radical scavenging activity of enzymatic hydrolysate of yak casein by different enzymes was studied. It was found that hydrolysates catalyzed by Alcalase showed the highest scavenging effect (p<0.05). Its scavenging effect varied with the hydrolysis time. When the hydrolysis time was 7 h, the obtained hydrolysate had the highest antioxidation property (p<0.05).Besides,the hydrolysis degree, TCA-soluble nitrogen index, short chain peptide content and protein recovery rate of casein hydrolysate by Alcalase for 7 h were very high, indicating a degradation of the most yak casein. In addition, the amino acid content of this hydrolysate was much lower than 15%.