Study on the Determination of Myricanol in Myrica esculenta Bark by HPLC
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Abstract:
The extraction conditions of myricanol in Myrica esculenta bark were studied and its content was determined by HPLC. The highest extraction rate of myricanol reached 4.78% by methanol extraction for 45 min. Gradient elution of the extract was performed on a Dima ODS (4.6 mm×250 mm, 5 µm) column using UV detector at 254 nm. A mixture of acetonitrile and water (80:20 by volume) was used as mobile phase at a flow rate of 1 ml/min. Within a linear range of 2.552~12.76 μg, the coefficient and RSD were of 1 and 100.1%, respectively. This method was simple, reliable and reproducible with highly sensitive. It can be used for the quality evaluation of Myrica esculenta resources for their further development.
