Application of Indirect ELISA Method for Rapid Detection of Vibrio parahaemolyticus
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Abstract:
The specific antibody was achieved by immune New Zealand white rabbit with V. parahaemolyticus antigen. Using this antibody, a indirect ELISA method for the detection of V. parahaemolyticus was developed with the best concentrations of the antibody, antigen and second antibody marked with HRP being of 107 cfu/mL, 1:4000, and 1:1000, respectively. The method can be used for the detection of V. parahaemolyticus in seafood with the detection limit and time of 104 cfu/mL and 8 h, respectively. However, the detection limit increased to 103 cfu/mL after an 8 h of bacteria multiplication. This rapid method had similar results with the conventional method, showing high practical application value.
