Abstract:The 16Sr DNA sequence of Acetobacter AC2005 was transferred into plasmid pMD18-T after amplified by PCR. Then the recombined plasmid was transformed in Escherichia coli. The analysis of 16S rDNA sequence indicated that the sequence was similar to the 16 Sr DNA sequence of Acetobactor pasteurianus with the homology up to 95%. According to the differences in biochemical characteristics, incubation characteristics, conservative sequence contrast, the strain was further identified as A. pasteurianus subsp.lovaniensis.
