Abstract:The antifatigue effects of L-carnitine in mutton were studied. The influences of L-carnitine on swimming time, body index, physiological and biochemical parameters in serum and L-Carnitine contents in leg muscle of Kunming mice were determined by parallel and cross experiments. The results showed that L-carnitine could prolong the average swimming time, lower body weight and abdomen fat, and decrease the contents of CHO, TC, urea nitrogen and lactic acid in serum. HPLC analysis showed that the L-carnitine contents correlated closely with the swimming time of the mice.