Analysis and Identification of Bacteria Population in Sufu Fermentation
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Abstract:
The variation of bacteria population in sufu fermentation was studied by molecular biology methods. The Genome DNA was extracted from the bacteria in Sufu sample, and its 16S rDNA fragment was amplified by PCR for further cloning and translating. The achieved 40 converters were examined by 16S rDNA PCR, 10 of which were false positive. Then the rest were sequenced bidirectionally for classifying them into different groups according to their homology. Meanwhile, comparison of these sequences with those on GeneBank by using Blast software showed that some genes had significant homology with foregone sequences in the GeneBank. This study enriched the knowledge of bacteria population distribution in sufu fermentation and revealed the relationship between microbe variation and Sufu fermentation.
