Preparation of Recombinant Antihypertensive Peptide by Trypsin-catalyzed Hydrolysis of Expressive Products of Engineering Bacteria
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Abstract:
Trysin-catalyzed hydrolysis of expressive products of recombinant E.coli BL21 was studied. Results showed that the best reaction temperature, pH, enzyme dosage and reaction time were 37 ℃, 8.0, 90 U/mL, and 4 h, respectively, under which the concentration of VLPVPR was up to 211.1 mg/L.
