Abstract:In this study, gardenia blue pigment was synthesized using amino acids and genipin which was produced via cellulose-catalyzed hydrolysis of geniposide. And the isolation and purification of the achieved pigment by ultra filtration were optimized by single factor test and orthogonal test. The results indicated that the most suitable amino acid, ratio of liquid to solid, the enzymatic hydrolysis time, molar ratio of cellulase to geniposide, molar ratio of amino acids to geniposide and reaction time were Glu, 8:1(v/w), 6 h, 1:8(g:g), 1:2(g:g), and 96 h, respectively. The best purification conditions were as follows: the retention molecular weight of 5000 Da, operating pressure of 0.5~0.8 MPa, room temperature and pH value of 7. Under those conditions, the gardenia blue pigment was achieved with high E590 nm1 cm (1%) value (≥192) and purity (≥95%).
