Optimization of HPLC Detection Conditions of Acid Polyphenols in Apple
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Abstract:
The detection conditions of acid polyphenols in apple by HPLC were optimized. Result showed that, the acid polyphenols in apple juice solution (pH value of 2.0) were well separated and detected on a C18 column (150×4.6 mm, 5 μm) at 20 ℃ with the UV detector at 320 nm. The mixture of methanol and acetic acid (4.3:5.7,V/V) was used as the best mobile phase at a flow rate of 1.00 mL/min.
